RNAimmuno - Delivery/Uptake

RNAimmuno - Delivery/Uptake

DharmaFECT
DMRIE-C
DOTAP
Effectene
FreeStyle MAX
Reagent
FuGENE 6
FuGENE HD
HiPerFect
INTERFERin
jetPEI
jetSi-ENDO
Lipofectamine
2000
Lipofectamine Ltx
Lipofectamine
RNAiMax
Lipofectin
Oligofectamine
Poly-L-arginine
(pLa)
Poly-L-lysine
(pLL)
siLentFect
siPORT Amine
siPort NeoFx
TransIT-TKO

Reagent name	DharmaFECT
Source	DharmaFect
Type of reagent	Lipid-based
Sample type	siRNA
Cell type	Mammalian cell lines
References	Mori T, Ishikawa A, Nemoto Y, Kambe N, Sakamoto M, Nakayama Y. Development of a novel nonviral gene silencing system that is effective both in vitro and in vivo by using a star-shaped block copolymer (star vector). Bioconjug Chem. 2009 Jun;20(6):1262-9.

Reagent name	DMRIE-C
Source	Invitrogen
Type of reagent	Lipid-based, liposomal formulation
Sample type	Plasmid DNA, RNA
Cell type	Mammalian Cells, Suspension cells Established Cell Lines, Primary Cells, Hard-to-Transfect Cells
References	Abul-Hassan K, Walmsley R, Boulton M. Optimization of non-viral gene transfer to human primary retinal pigment epithelial cells. Curr Eye Res. 2000 May;20(5):361-6. Wong AW, Scales SJ, Reilly DE. DNA internalized via caveolae requires microtubule-dependent, Rab7-independent transport to the late endocytic pathway for delivery to the nucleus. J Biol Chem. 2007 Aug 3;282(31):22953-63. Uchida E, Mizuguchi H, Ishii-Watabe A, Hayakawa T. Comparison of the efficiency and safety of non-viral vector-mediated gene transfer into a wide range of human cells. Biol Pharm Bull. 2002 Jul;25(7):891-7. Gebhart CL, Kabanov AV Evaluation of polyplexes as gene transfer agents. J Control Release. 2001 Jun 15;73(2-3):401-16. Kaiser S, Toborek M. Liposome-mediated high-efficiency transfection of human endothelial cells. J Vasc Res. 2001 Mar-Apr;38(2):133-43. Masotti A et al. Comparison of different commercially available cationic liposome-DNA lipoplexes: Parameters influencing toxicity and transfection efficiency. 2009 Feb 1;68(2):136-44.

Reagent name	DOTAP
Source	Roche, Sigma-Aldrich
Type of reagent	Liposome formulation of the cationic lipid DOTAP ( N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate
Sample type	Plasmid DNA, RNA, RNP, oligonucleotides
Cell type	Eukaryotic cells
References	Uchida E, Mizuguchi H, Ishii-Watabe A, Hayakawa T. Comparison of the efficiency and safety of non-viral vector-mediated gene transfer into a wide range of human cells. Biol Pharm Bull. 2002 Jul;25(7):891-7. Masotti A et al. Comparison of different commercially available cationic liposome-DNA lipoplexes: Parameters influencing toxicity and transfection efficiency. J Biomed Biotechnol. 2009 Feb 1;68(2):136-44. Martino S, di Girolamo I, Tiribuzi R, D'Angelo F, Datti A, Orlacchio A. Efficient siRNA delivery by the cationic liposome DOTAP in human hematopoietic stem cells differentiating into dendritic cells. J Biomed Biotechnol. 2009;2009:410260. Kornek M et al. 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP)-formulated, immune-stimulatory vascular endothelial growth factor a small interfering RNA (siRNA) increases antitumoral efficacy in murine orthotopic hepatocellular carcinoma with liver fibrosis. Mol Med. 2008 Jul-Aug;14(7-8):365-73. Simberg D, Weisman S, Talmon Y, Barenholz Y. DOTAP (and other cationic lipids): chemistry, biophysics, and transfection. Crit Rev Ther Drug Carrier Syst. 2004;21(4):257-317.

Reagent name	Effectene
Source	Invitrogen Sigma-AldichQiegen
Type of reagent	Lipid-based, non-liposomal
Sample type	DNA
Cell type	Primary and established cell lines
References	Tschuch C et al., Off-target effects of siRNA specific for GFP. BMC Mol Biol. 2008 Jun 24;9:60. Liou JY et ala., An efficient transfection method for mouse embryonic stem cells. Methods Mol Biol. 2010;650:145-53.

Reagent name	FreeStyle MAX Reagent
Source	Invitrogen
Type of reagent	Lipid-based; Cationic-lipid formulation
Sample type	Plasmid DNA
Cell type	Established Cell Lines
References	Liu C, Dalby B, Chen W, Kilzer JM, Chiou HC. Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells. Mol Biotechnol. 2008 Jun;39(2):141-53.

Reagent name	FuGENE 6
Source	Roche
Type of reagent	Lipid-based, non-liposomal
Sample type	DNA
Cell type	Eukaryotic primary and and established cell lines, Hard-to-Transfect Cells
References	Arnold AS et al. Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500. Fundam Clin Pharmacol. 2006 Feb;20(1):81-9. Jacobsen LB, Calvin SA, Colvin KE, Wright M. FuGENE 6 Transfection Reagent: the gentle power. Methods. 2004 Jun;33(2):104-12. Ebadi P et al. The efficiency of CD40 down regulation by siRNA and antisense ODN: comparison of lipofectamine and FuGENE6. Iran J Immunol. 2009 Mar;6(1):1-11. Masotti A et al. Comparison of different commercially available cationic liposome-DNA lipoplexes: Parameters influencing toxicity and transfection efficiency. Colloids Surf B Biointerfaces. 2009 Feb 1;68(2):136-44. Djurovic S, Iversen N, Jeansson S, Hoover F, Christensen G. Comparison of nonviral transfection and adeno-associated viral transduction on cardiomyocytes. Mol Biotechnol. 2004 Sep;28(1):21-32. Lee MJ et al. Intraperitoneal gene delivery mediated by a novel cationic liposome in a peritoneal disseminated ovarian cancer model. Gene Ther. 2002 Jul;9(13):859-66. Hellgren Iet al. Highly efficient cell-mediated gene transfer using non-viral vectors and FuGene6: in vitro and in vivo studies. Cell Mol Life Sci. 2000 Aug;57(8-9):1326-33. Orth P, Weimer A, Kaul G, Kohn D, Cucchiarini M, Madry H. Analysis of novel nonviral gene transfer systems for gene delivery to cells of the musculoskeletal system. Mol Biotechnol. 2008 Feb;38(2):137-44.

Reagent name	FuGENE HD
Source	Roche
Type of reagent	Lipid-based, non-liposomal
Sample type	DNA
Cell type	Eukaryotic primary and and established cell lines including insect cells, Hard-to-Transfect Cells, Stem cells
References	Yalvac ME, Ramazanoglu M, Gumru OZ, Sahin F, Palotás A, Rizvanov AA. Comparison and optimisation of transfection of human dental follicle cells, a novel source of stem cells, with different chemical methods and electro-poration. Neurochem Res. 2009 Jul;34(7):1272-7. Gheisari Y, Soleimani M, Azadmanesh K, Zeinali S. Multipotent mesenchymal stromal cells: optimization and comparison of five cationic polymer-based gene delivery methods. Cytotherapy. 2008;10(8):815-23. Liu J, Jones KL, Sumer H, Verma PJ. Stable transgene expression in human embryonic stem cells after simple chemical transfection. Mol Reprod Dev. 2009 Jun;76(6):580-6. Dickens S et al. Nonviral transfection strategies for keratinocytes, fibroblasts, and endothelial progenitor cells for ex vivo gene transfer to skin wounds. Tissue Eng Part C Methods. 2010 Dec;16(6):1601-8.

Reagent name	HiPerFect
Source	Qiegen
Type of reagent	Lipid-based
Sample type	siRNA, miRNA
Cell type	Eukaryotic cells
References	Kumar S et al., Intracranial administration of P gene siRNA protects mice from lethal Chandipura virus encephalitis. PLoS One. 2010 Jan 7;5(1):e8615. Nabzdyk CS et al. High throughput RNAi assay optimization using adherent cell cytometry. J Transl Med. 2011 Apr 25;9:48.

Reagent name	INTERFERin
Source	Polyplus transfection
Type of reagent	-
Sample type	siRNA
Cell type	Primary and and established cell lines
References	Alshamsan A, Haddadi A, Incani V, Samuel J, Lavasanifar A, Uludag H. Formulation and delivery of siRNA by oleic acid and stearic acid modified polyethylenimine. Mol Pharm. 2009 Jan-Feb;6(1):121-33. Semizarov D, Frost L, Sarthy A, Kroeger P, Halbert DN, Fesik SW. Specificity of short interfering RNA determined through gene expression signatures. Proc Natl Acad Sci U S A. 2003 May 27;100(11):6347-52. Epub 2003 May 13.

Reagent name	jetPEI
Source	Polyplus transfection
Type of reagent	Polymer-based
Sample type	DNA
Cell type	Primary and and established cell lines
References	Anderson DG et al. A polymer library approach to suicide gene therapy for cancer. Proc Natl Acad Sci U S A. 2004 Nov 9;101(45):16028-33. Alshamsan A, Haddadi A, Incani V, Samuel J, Lavasanifar A, Uludağ H. Formulation and delivery of siRNA by oleic acid and stearic acid modified polyethylenimine. Mol Pharm. 2009 Jan-Feb;6(1):121-33. Van Vliet LD, Chapman MR, Avenier F, Kitson CZ, Hollfelder F. Relating chemical and biological diversity space: a tunable system for efficient gene transfection. Chembiochem. 2008 Aug 11;9(12):1960-7. Orth P, Weimer A, Kaul G, Kohn D, Cucchiarini M, Madry H. Analysis of novel nonviral gene transfer systems for gene delivery to cells of the musculoskeletal system. Mol Biotechnol. 2008 Feb;38(2):137-44.

Reagent name	jetSi-ENDO
Source	Polyplus transfection
Type of reagent	-
Sample type	siRNA, co-transfection with plasmid DNA
Cell type	Mammalian cells
References	Bøe S, Longva AS, Hovig E. Photochemically induced gene silencing using small interfering RNA molecules in combination with lipid carriers. Oligonucleotides. 2007 Summer;17(2):166-73.

Reagent name	Lipofectamine 2000
Source	Invitrogen
Type of reagent	Lipid-based
Sample type	Plasmid DNA, RNAi reagents (siRNA)
Cell type	Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
References	Clements BA, Incani V, Kucharski C, Lavasanifar A, Ritchie B, Uludağ H. A comparative evaluation of poly-L-lysine-palmitic acid and Lipofectamine 2000 for plasmid delivery to bone marrow stromal cells. Biomaterials. 2007 Nov;28(31):4693-704. Dalby B et al. Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications. Mol Biotechnol. 2004 Jun;33(2):95-103. Gheisari Y, Soleimani M, Azadmanesh K, Zeinali S. Multipotent mesenchymal stromal cells: optimization and comparison of five cationic polymer-based gene delivery methods. Cytotherapy. 2008;10(8):815-23. Ebadi P et al. The efficiency of CD40 down regulation by siRNA and antisense ODN: comparison of lipofectamine and FuGENE6. Iran J Immunol. 2009 Mar;6(1):1-11. Djurovic S, Iversen N, Jeansson S, Hoover F, Christensen G. Comparison of nonviral transfection and adeno-associated viral transduction on cardiomyocytes. Mol Biotechnol. 2004 Sep;28(1):21-32. Orth P, Weimer A, Kaul G, Kohn D, Cucchiarini M, Madry H. Analysis of novel nonviral gene transfer systems for gene delivery to cells of the musculoskeletal system. Mol Biotechnol. 2008 Feb;38(2):137-44. Martino S, di Girolamo I, Tiribuzi R, D'Angelo F, Datti A, Orlacchio A. Efficient siRNA delivery by the cationic liposome DOTAP in human hematopoietic stem cells differentiating into dendritic cells. J Biomed Biotechnol. 2009;2009:410260.

Reagent name	Lipofectamine Ltx
Source	Invitrogen
Type of reagent	Lipid-based
Sample type	Plasmid DNA
Cell type	Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
References	Wong AW, Scales SJ, Reilly DE. DNA internalized via caveolae requires microtubule-dependent, Rab7-independent transport to the late endocytic pathway for delivery to the nucleus. J Biol Chem. 2007 Aug 3;282(31):22953-63. Hattori Y, Maitani Y Low-molecular-weight polyethylenimine enhanced gene transfer by cationic cholesterol-based nanoparticle vector. Biol Pharm Bull. 2007 Sep;30(9):1773-8. Martino S, di Girolamo I, Tiribuzi R, D'Angelo F, Datti A, Orlacchio A. Efficient siRNA delivery by the cationic liposome DOTAP in human hematopoietic stem cells differentiating into dendritic cells. J Biomed Biotechnol. 2009;2009:410260.

Reagent name	Lipofectamine RNAiMax
Source	Invitrogen
Type of reagent	Lipid-based
Sample type	RNAi (Synthetic siRNA)
Cell type	Stem Cells,> Primary Cells, Hard-to-Transfect Cells, Established Cell Lines
References	Zhao M et al. Lipofectamine RNAiMAX: an efficient siRNA transfection reagent in human embryonic stem cells. Mol Biotechnol. 2008 Sep;40(1):19-2 Nabzdyk CS et al. High throughput RNAi assay optimization using adherent cell cytometry. J Transl Med. 2011 Apr 25;9:48.

Reagent name	Lipofectin
Source	Invitrogen
Type of reagent	Lipid-based
Sample type	RNAi reagnts (Synthetic siRNA)
Cell type	Primary Cells, Established Cell Lines, Hard-to-Transfect Cells
References	Omidi Y et al. Toxicogenomics of non-viral vectors for gene therapy: a microarray study of lipofectin- and oligofectamine-induced gene expression changes in human epithelial cells. J Drug Target. 2003 Jul;11(6):311-23.

Reagent name	Oligofectamine
Source	Invitrogen
Type of reagent	Lipid-based
Sample type	siRNA, oligonuclotides
Cell type	Primary Cells Hard-to-Transfect Cells
References	Omidi Y, Hollins AJ, Benboubetra M, Drayton R, Benter IF, Akhtar S. Toxicogenomics of non-viral vectors for gene therapy: a microarray study of lipofectin- and oligofectamine-induced gene expression changes in human epithelial cells. J Drug Target. 2003 Jul;11(6):311-23. Omidi Y, Barar J, Heidari HR, Ahmadian S, Yazdi HA, Akhtar S. Microarray analysis of the toxicogenomics and the genotoxic potential of a cationic lipid-based gene delivery nanosystem in human alveolar epithelial a549 cells. Toxicol Mech Methods. 2008;18(4):369-78. Merkerova M, Klamova H, Brdicka R, Bruchova H. Targeting of gene expression by siRNA in CML primary cells. Mol Biol Rep. 2007 Mar;34(1):27-33.

Reagent name	Poly-L-arginine
Source	Sigma-Aldrich
Type of reagent	Polycationic polypeptide
Sample type	Plasmid DNA, RNA oligonuclotids, siRNA
Cell type	-
References	Koo H, Kang H, Lee Y. Analysis of the Relationship between the Molecular Weight and Transfection Efficiency/Cytotoxicity of Poly-L-arginine on a Mammalian Cell Line. Bull. Korean Chem. Soc. 2009, Vol. 30, No. 4

Reagent name	Poly-L-lysine (pLL)
Source	Sigma-Aldrich
Type of reagent	Polycationic polypeptide
Sample type	Plasmid DNA, siRNA
Cell type	Eukaryotic cells
References	Koo H, Kang H, Lee Y. Analysis of the Relationship between the Molecular Weight and Transfection Efficiency/Cytotoxicity of Poly-L-arginine on a Mammalian Cell Line. Bull. Korean Chem. Soc. 2009, Vol. 30, No. 4

Reagent name	siLentFect
Source	Bio-Rad
Type of reagent	-
Sample type	siRNA, co-transfection with plasmid DNA
Cell type	Cell lines
References	Kodym R, Kodym E, Story MD. 2'-5'-Oligoadenylate synthetase is activated by a specific RNA sequence motif. Biochem Biophys Res Commun. 2009 Oct 16;388(2):317-22. Epub 2009 Aug 5.

Reagent name	siPORT Amine
Source	Ambion
Type of reagent	Polyamine-based formulation
Sample type	small RNAs
Cell type	non-primary mammalian cells
References	Martino S, di Girolamo I, Tiribuzi R, D'Angelo F, Datti A, Orlacchio A. Efficient siRNA delivery by the cationic liposome DOTAP in human hematopoietic stem cells differentiating into dendritic cells. J Biomed Biotechnol. 2009;2009:410260. Grayson AC, Ma J, Putnam D.L Kinetic and efficacy analysis of RNA interference in stably and transiently expressing cell lines. Mol Pharm. 2006 Sep-Oct;3(5):601-13. Merkerova M, Klamova H, Brdicka R, Bruchova H. Targeting of gene expression by siRNA in CML primary cells. Mol Biol Rep. 2007 Mar;34(1):27-33.

Reagent name	siPort NeoFx
Source	Ambion
Type of reagent	lipid-based
Sample type	siRNA, miRNA, small RNAs
Cell type	Cell lines
References	Ceballos C et al. Cationic nucleoside lipids based on a 3-nitropyrrole universal base for siRNA delivery. Bioconjug Chem. 2009 Feb;20(2):193-6. Martino S, di Girolamo I, Tiribuzi R, D'Angelo F, Datti A, Orlacchio A. Efficient siRNA delivery by the cationic liposome DOTAP in human hematopoietic stem cells differentiating into dendritic cells. J Biomed Biotechnol. 2009;2009:410260.

Reagent name	TransIT-TKO
Source	Mirus
Type of reagent	polymer/lipid formulation
Sample type	siRNA
Cell type	siRNA Primary and and established cell lines
References	Awasthi S, Cox RA. Transfection of murine dendritic cell line (JAWS II) by a nonviral transfection reagent. Biotechniques. 2003 Sep;35(3):600-2, 604. Zhang M, Bai CX, Zhang X, Chen J, Mao L, Gao L. Downregulation enhanced green fluorescence protein gene expression by RNA interference in mammalian cells. RNA Biol. 2004 May;1(1):74-7. Andersen MØ, Howard KA, Paludan SR, Besenbacher F, Kjems J. Delivery of siRNA from lyophilized polymeric surfaces. Biomaterials. 2008 Feb;29(4):506-12. Epub 2007 Oct 24. Ghonaim HM, Li S, Blagbrough IS. Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery. Pharm Res. 2009 Jan;26(1):19-31.